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Airlift bioreactors-fermentors

200L 2000L Double mechanical seal airlift fermentor system for microoganism

The system is for microoganism cultivation.

  • Description
  • Competitive & Advantage
  • Specifications
  • Applications
  • Inquiry
Description

1. Description for 200L and 2000L Mechanical Agitation Fermenter  

 Volume  Parameter 

200L fermenter with agitation

2000Lfermenter with agitation

TTL Volume

206L

2004L

Work Volume

155L

1503L

Tank Diameter

450mm

1000mm

Tank Height

1200mm

2360mm

The ratio of D:H

1:2.66

1:2.36

Agitator-motor (SEW)

Germany SEW AC electrical machine.Speed range:50-400rpm±%1FS

Sterilization mode

Have complete and reliable SIP sterilization system, the sterilization temperature and process is fully automatic monitored by computer sterilization program; Sterilization time is calculated by software automatically and remind after sterilization, thereby to reach safe, convenient and rapid sterilization process.  Sterilization temperature: 100-130℃.

Tank body

1. Tank material : SUS316L  Jacket material:SUS304 

2. The polishing precision: Ra0.4µm,

The roughness of electropolishing internal surface reach to Ra≤0.4µm. The fillet is formed by Japanese plate hydraulic forming. All nozzles are formed by Japanese tensile method to form.  They confirm to GMP and FDA standard.

All elliptical heads are designed according to Japan Standard.

Tank pressure

Designed Pressure≤0.25MpaWork pressure ≤0.2Mpa

Jacket pressure

Designed Pressure≤0.35MpaWork pressure ≤0.3Mpa

Speed regular (VFD)

SIEMENS

Mechanical seal

Germany EagleBurgmann double mechanical seal

Stirring oar

2 tiered 6 flat-bladed impeller, adjustable .

Foam breaker

1 tier mechanical foam breaker impeller, adjustable

Filtration system

Maximum ventilation capacity is designed at 1.5V/VM, the precision filter applies 0.2μm sterilizing membrane filter of Sartorius brand imported from Germany which can meet repeated high temperature sterilization.

Ventilation pressure

 Manual control the ventilatory by pressure adjustment; indicate the air inflow capacity by rotameter.

Tail gas discharging

Efficiently protected from biological pollution by tail gas filter

Culture transferring and inoculation

 Adopt regular flame inoculation is much safe and aseptic through peristaltic pump, sanitary silicone tube and exclusive plug pins.

Probe ports

4 ports1 ph port and 3 extra ports with blind plugs)

Competitive Advantage

1. Control System for Biological Process

1) System Summary

BIO-4000 industrial control system is researched and developed by our company. Adopt PLC as the control unit to acquire data on site and transfer to the touch screen and PC by RS-485 signal , to realize the parameter setting, sequence control, process control and a comprehensive analysis of the various data, automatic formation of statements, control interface, touch screen direct display of the implementing agencies working conditions and parameters. It enhances the scalability and compatibility of the system because the standard MODBUS protocol port.

Lower Computer SystemAdopt PLC as the control unit commands of touch screen or PC are interpreted to corresponding timing signal to directly control the corresponding equipment. Besides, equipment condition data can be converted into digital signal, and make feedback to the touch screen display or PC for further data processing.

l The software control part adopts PID close loop, which has the features of control High precision control, and the low energy consumption.

l The touch screen as a senior control source, possess the functions of password classification management, parameter setting, alarm management, real-time curve display and on-site report.

Specifications

1) Control System 

NO. 

CONTROL PARAMETER 

CONTROL INDICATION 

1

PH

  • 1. PLC automatic control
  • 2. Apply intellectual expert PID control, with very good control function of PH degree. It is equipped with Switzerland Hamilton gel type exclusive high temperature resistant sensor.
  • Display range: 0.00-14.00ph, control accuracy: ±0.02, resolution ratio:0.01;
  • Automatic acid and alkali feeding, can be fed by PLC automatic accurate control peristaltic pump or manually to ensure the security in material transmission in fermentation.
  • 33. Touch screen display
    • Display content (PH set value, ph real value, auto curve, alarm parameter, acid & alkali feeding peristaltic pump condition)

3

Temperature

  • 1. PLC automatic control, PT100 temperature sensor.
  • 2. Touch screen display
  • Display content (temperature set value, temperature real value, auto curve, alarm parameter, jacket temperature, all working parts running or stop condition, etc.)  Temperature up and lower limit set, control accuracy: ±0.2℃; resolution ratio: 0.1℃; test range: 0-160℃.
  • 3. Automatic heating up by electric heating; cooling water automatic cooling down by solenoid valve control. (Denmark Grundfos circulating pump).

4

Speed

  • 1. PLC automatic control
  • 2. Applies AC SEW motor and Simens Inverter.
  • 3. Rotating speed range: 
  •    200L: 50-450 rpm, ±%1FS 
  •   2000L: 50-450 rpm, ±%1FS  
  • 4. Touch screen display
  • Display content ( rotating speed set value, real value, auto        curve, alarm parameter)

5

Defoaming

  • 1. PLC automatic control
  • 2. Defoamed by efficient mechanical pressing type and liquid level electrode automatic control. The defoaming system is consisted of feeding hole, conveying pipelines, steam sterilization system, peristaltic pump and PLC to ensure the security in material transmission in fermentation. Add defoamer manually is also feasible. Liquid level electrode automatic control defoaming sensitivity: 100-100000Ω..
  • 3. Touch screen display
  • Display content (defoaming sensor and defoaming peristaltic pump working condition)

6

Material feeding

  • 1. PLC automatic control
  • 2. Digital set time-lapse and quantitive control, material feeding quantity display and record, easy for calculation.

1. Main Units For Fermentation System  

NO. 

NAME 

Original 

Remark 

1

Tank

China

 

2

Agitator-motor (SEW) 

Germany brand, China made 

SEW  

3

Agitation System (axis,oar,etc)

China

 

4

Double mechanical seal

Germany

EagleBurgman

5

Viewing Glass, lamp

China

 

6

Steam flushed sanitary sampling Valve

China

 

7

Tank bottom diaphragm vavle

China

 

8

Cabinet, module, transmitter, cable, switch and so on

China

 

9

HMI (MCGSE)

China

HMI

10

PLC

Germany brand, China made

SEIMENS, S7-200

11

PH probe 

Swiss 

Hamilton origin 

12

Liquid Level probe

China

 

13

Defoaming probe

China

 

14

Feeding pin and pin cap

China

 

15

Glass Bottle

China

 

16

Silicon Hose

China

 

17

Circulating Pump

Grandfos, China made

 

18

Filter 

Germany 

SARTORIUS  

19

Electric Heater

China

 

20

Peristaltic pump

China

Foam, acid, alkali, molasses (feeding)

21

Solenoid valve for cooling water inlet

China Taiwan

 

22

Air Rotor Flow meter

China

 

23

Stop valve

China

 

24

Check Valve

China

Cooling, jacket in

25

 Air rebreather, acro 37 (Glass bottle) 

Germany 

Sartorius  

26

Pressure Indicator

China

 

29

Converter (VFD) for agitator

Germany brand China Made

SIEMENS

30

Manual diaphragm Valve

China

 

31

Temperature PT100 ( Tank body)

Japan Chip, China assemble

 

32

Temperature PT100 (heater)

Japan Chip, China assemble

 

33

O -RING (silicone gel)

China

 


Applications

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Microbial
becteria culture 
Yeast
Enzyme
Fungi
Bioprocessing Cultivation
Microorganisms
lactic acid bacteria , lactobacillus, bacterium acidi lactici
rhizobium ,nodule bacteria, legume bacteria
Microbial fermentation
Microbial Fermentation Manufacturing Capabilities
 organic fertilizer
Biotics (Fermentation bacteria : Bacillus sp, Bacillus subtilis...)
ultraclean proteins
the gut bacterium Escherichia coli
the yeast Pichia pastoris
probiotic
lysine                     
insulin
leben
cultivate Bacillus subtilis, Laccto bacillus….
bio fertilizer fermenter
biologic ph fertilizer
Apple cider
bio fertilizer 
waste water
fusarium sambucinum
enzyme preparation
Bio Pesticide Fermentors
biological pesticides
lactic acid bacteria
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