Stainless steel bioreactors-fermentors

In-Situ Sterilizable Bioreactors 500L

Bacteria Fermenter with double mechanical Seal at bottom

  • Description
  • Competitive & Advantage
  • Specifications
  • Inquiry

Fermentor|bioreactor(500Liters ,Mechanical sealing system at the bootom of the fermenter;Double mechanical Seal) Model:KNIKBIO -500SBA

Total volume:500 liters

Working volume: 150L -400 liters
Material: 316L stainless steel with material contact to the other 304 stainless steel

Sterilization method: Manual situ sterilization(SIP  options)
Control system: Siemens PLC, industrial LCD touch computer control, parameters can be set to store, can be remotely connected to the computer through the USB interface, the data

Working volume 250 to 400 liters and The main material of the fermenter is made by 316Lstainless steel. The design pressure: P(inside)= 0.3Mpa, P(interlayer)= 0.3Mpa, The polishing precision: Ra0.4~0.6, the ratio between diameter and height: 1:2.6

Stirring system
Mechanical sealing system at the top of the fermenter; high-powered stirring oar, antifoam oar; AC electrical machine: infinite speed variation; rpm in the fermenter: 70~500rpm±1%,The height of the stirring oar can be adjusted, too.

Temperature detection and control
Heat by thermostatic water tank and circulate by circulating pump(Grundfos, Denmark), autocontrol (cooling water+5℃)~65℃±0.1℃,temperature probe(US)

pH detection and control
2.00-12.00±0.05pH, autocontrol by adding acid and base, pH sensor (Mettler, Switzerland) and shielding lead (Mettler, Switzerland). fermenter is autocontrolled with peristaltic pump

DO detection and control
0-150±3% displaying precision 0.1%, sensor (Mettler, Switzerland) and shielding lead(Mettler, Switzerland)

Feed control
fermenter uses peristaltic pump (Longer) to add meterials

Antifoam control
Tested by sencor and added antifoamer by peristaltic pump

Intake control (air): manual control rotameter

pressure detection and control
Pressure Control: Pressure meter and digital display and digital remote control, pressure control

we are compliant fermentation equipment for research, development, pilot and production applications. Our Factory in China. We provide the fermentor products including : Stainless steel  fermenter, Pilot combo stainless steel fermentor, glass fermentor,  fermenter, Multi-Union fermenter,Multi-stagefermentation tank,and shaking Incubators ,  Positive join stainless steel fermentor.

Competitive Advantage

Technical parameter:
Material and brand of key components




Key component


Material and brand


Vessel system

The cylinder designed by our company uses stainless steel


Speed Motor



Speed regulator

Fuji, Japan


Solenoid valve



Aeration filter

0.2μm, Dominica United Kingdom


Breathing filter

0.2μm, SARTORIUS, Germany


pH electrode

Hamilton /Mettler-Toledo, Switzerland


DO electrode

Hamilton /Mettler-Toledo, Switzerland 


Peristaltic pump

Lange (United States)



Siemens Germany


LCD touch screen



Mechanical seal

John Crane United Kingdom


1. Double end face and cooling lubricaton system        7. Double mechanical seal - Double protection

2. Inactivated exhaust system                                    8. Pressure regulator

3. Effect of stirring                                                     9. Balance feeding weighing system

4. Thermal mass flowmeter                                         10. Tank weighing module-mettler

5. Tank cap automatically open system                       11. Siemens PLC, schneider intermediate

6. Tank bottom feeding and moving system                 12. All kinds of impeller


For cell cultivation, bacteria, microbial fermentation and etc.
SEQ. main features
1 Full automatic equipment system including automatic cleaning (CIP), automatic sterilzation (SIP)
2 Flexible and modular-designed systemuser-friendly software, ease of operation
3 With rich experience for customers from different countries with mature standard design and optimized customized design
4 Economical choice and guarantee a trouble free operation and global
5 Efficient service support and spare part availability for overseas and domestic market


Type BLBIO-5/7/10SJ BLBIO-20/30/50 BLBIO-100/150/200SJ BLBIO-250/300/500SJ
Total volume 5/7/10 20/30/50 100/150/200 250/300/500
Maximum loading Amount of liquid 4/5/8 16/21/40 70/120/160 200/210/400
Materials 316L stainless steel
Stirring Methods the magnetic structure and top driven by the mechanical coupling
Basic Configuration (optional) temperature, RPM, PH, DO, antifoam,feed , aeration (manual), Pressure (manual), sip
Extensible Configuration (optional) Liquid level control, feed weighing system, multifeeds & online testing of the content of methanol and ethanol, detection of O2 & CO2 exhausts
Control System BLBIO-B. C. S BIO-Process controller
Electric Power 220V 2KW 220V 3KW 380V 6KW 380V 8KW
Dimension(L*W*H) 890*660*1600 1300*790*1700 1400*820*2200 2000*1500*2800
Net weight (kg) 120 150 250 400
The amount of steam for sterilization 220V 3-6KW 380V 9-12KW 380V 18-24KW 380V 36-45KW
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